Nucleosides-based identification model for Fritillariae Cirrhosae Bulbus / 中国中药杂志

A high performance liquid chromatography( HPLC) method was established for the fast,and precise determination of ten nucleosides in Fritillariae Cirrhosae Bulbus and its counterfeits. Then multivariate statistical analyses,such as clustering analysis,principal component analysis( PCA),and Fisher' s linear discriminant analysis( LDA),were conducted to establish a discriminant function model for an integrated analysis. The results indicated that data acquisition time of a single sample was shortened within 16 min by the HPLC method. In the range of 5-1 000 mg·kg~(-1),the mass concentrations of all nucleosides exhibited good linear relationships with the corresponding peak areas( R2> 0. 999). The spiked recoveries were in the range of 93. 83%-108. 9% with RSDs of0. 12%-1. 3%( n = 5). The limit of quantitation( LOQ) was 0. 98-4. 13 mg·kg~(-1). As revealed by the clustering analysis,Fritillariae Cirrhosae Bulbus and the counterfeits could be discriminated into two clusters based on the content of nucleosides. Fisher's LDA could achieve this discrimination,while PCA dimension reduction failed. The accuracy of the discriminant function model established on the screened characteristic indicators reached 97. 5%. The present study proposed a new identification method of Fritillariae Cirrhosae Bulbus with one-dimensional indicators,which is simple,accurate,and reliable. It can provide a scientific basis for further optimizing the identification techniques for Fritillariae Cirrhosae Bulbus and inspiration for quality control strategy development of Chinese medicinal materials.

Multi-effect evaluation of community comprehensive intervention against osteoporosis / 上海预防医学

Objective:This study was to evaluate the effects of comprehensive intervention， at different stages， in community osteoporosis patients. Method:Osteoporosis intervention was set up for years in a follow-up cohort community， in which patients with primary osteoporosis who volunteered to participate in the research were divided into control and intervention groups. The latter received comprehensive intervention consisting of physical therapy with osteoporosis therapeutic instrument， treatment with the prescription of strengthening waist and keeping bones in combination with calcitriol， health Qigong and changing tendon exercise， and health education lectures. The therapeutic effect was assessed at three different stages： prior to intervention， 3 and 6 months after intervention. The effect indicators included the following： visual anologue scale （VAS） pain score， clinical symptom total score， general condition total score， bone density and bone metabolism. Results:VAS pain index， total clinical symptom score and total systemic condition score in the intervention group were significantly lower than those in the control group （P<0.05）. Bone density in the intervention group increased at 6 months and the difference was statistically significant （P<0.05）， compared with the control group （P<0.05）. All the four bone biochemical indexes in the intervention group changed compared with those before intervention， and the improvement of PINP， β-CTX， 25（OH）D in the intervention group was better than that in the control group. Conclusion:Result of effect evaluation with multiple indicators demonstrates that comprehensive intervention is suitable for promotion in prevention and treatment of primary osteoporosis in community.

Multiple Organ Echinococcosis:Report of One Case and Literature Review / 中国医学科学院学报

A patient with multiple-organ echinococcosis suffered from liver echinococcosis,lung echinococcosis,and pelvic echinococcosis successively in the past three decades.From the first operation at 19 years-old,she underwent operations several times due to the recurrence of multiple organ involvement.Echinococcosis is a zoonotic disease.Although the liver usually is the primary site,the disease can also invade many other organs.Diagnosis is typically based on disease history and imaging findings.Thorough removal of the lesions during the first operation is particularly important.Comprehensive evaluations and multi-disciplinary team are helpful in the treatment of patients with multiple organ invasion.

Correlation between Methylation of SALL3 and Cervical Cancer / 中国医学科学院学报

Objective To detect the methylation status of SALL3 gene promoter region in normal cervical tissues,cervical cancer tissues,and cervical cancer cell lines and thus explore the relationship between methylation status and the expression of SALL3 gene.Methods The DNA methylation statuses of SALL3 gene in normal cervical,cervical cancer tissues and cervical cancer cell lines were analyzed by methylation-specific PCR(MS-PCR).The expressions of SALL3 mRNA in cervical cancer cell lines,cervical cancer tissues,and normal cervical tissues were detected by RT-PCR.Results In cervical cancer and matched peri-carcinomatous samples,the methylation levels of SALL3 were up-regulated(CCa .CCap:=0.046;CCa .NC =0.039)and the protein expressions were down-regulated(CCa .CCap:=0.012;CCa .NC =0.000)when compared with normal cervix samples.The mRNA levels of SALL3 in HeLa and SiHa cells treated with 5-Azacytidine were elevated in a dose-dependent manner(HeLa:=0.001;SiHa:=0.002).The methylation level of SALL3 was higher in high risk human papillomavirus(HPV)-positive cervical samples than in HPV-negative cervical samples(=0.014),which also resulted in a descending SALL3 expression in HPV-positive samples(=0.021).Conclusions The hypermethylation of SALL3 in promoter regions inhibits the expression of SALL3 in cervical cancer tissue samples.Infection with high-risk HPV serotypes may increase the methylation of SALL3 promoter region,silence its expression,and thus promote the development of cervical cancer.

Mechanical properties and cytocompatibility of a new-type nano-bionic anti-adhesion hernia mesh / 中国组织工程研究

BACKGROUND:A new-type nano-bionic anti-adhesion hernia mesh was developed in our previous research.OBJECTIVE:To investigate the mechanical properties and cytocompatibility of the new-type nano-bionic anti-adhesion hernia mesh.METHODS:The tensile strength of the compound hernia mesh was detected using a textile detector.Mouse fibroblasts (L929) were cultured with the compound hernia mesh,and cell structures on the mesh surface were observed under electron microscope at 1,3,5 days after culture.In addition,L929 cells were co-cultured with compound hernia mesh,polypropylene patch,and polyester patch,respectively.Cells cultured alone were used as negative controls.After 1,3,5 days of culture,MTS array was used to detect cell proliferation and evaluate cytotoxicity;after 3 days of culture,western blot was used to detect the content of type Ⅰ and Ⅲ collagens.RESULTS AND CONCLUSION:The average tensile strength of the compound hernia mesh was 31.2 N The number of fibroblasts on the nanofibrous layer of the compound hernia mesh increased as long as cultured.The cells spread along the nanofibers and pseudopodia extended from the cells formed polygon and fusiform structures,with a good cross-linking with the mesh.A complete cell layer covered all pores of the nanofibers at 5 days.The cytotoxicity of the nanofibrous layer of the compound hernia mesh was graded 0,and the cytotoxicity was graded 1 of polypropylene and polyester patches.All the three kinds of patches fulfilled the implantation requirements,and the compound hernia mesh had better biological properties.No significant differences were found among groups in the contents of type Ⅰ and Ⅲ collagens at 3 days of culture.To conclude,the new-type nano-bionic anti-adhesion hernia mesh has good mechanical properties and cytocompatibility.

Effect of corpus luteum blood flow measured by three-dimensional power Doppler ultrasound on pregnancy in elderly women / 西安交通大学学报(医学版)

Objective To explore the effect of corpus luteum blood flow measured by three-dimensional power Doppler ultrasound on pregnancy in elderly women.Methods We selected 464 elderly women with normal ovulation who came to the First Affiliated Hospital of Xi'an Jiaotong University from January 2016 to July 2017 as study group.Another 436 women aged less than 35 years old who planned pregnancy in the same period were selected as control group.The clinical data were compared between the two groups.Results There was no significant difference in average diameter of dominant follicles and endometrial thickness at each monitoring point between the two groups (P＞0.05).There was no significant difference in corpus luteum blood flow grade 1 day after ovulation (P＞0.05),but corpus luteum blood flow grade 7 to 9 days after ovulation in study group was significantly reduced as compared with control group (P＜0.05).The two groups did not differ significantly in corpus luteum volume (V)or vascular parameters 1 day after ovulation,corpus luteum V 7-9 days after ovulation (P＞0.05).However,luteum vascularization index (VI),flow index (FI)and vascularization flow index (VFI) significantly decreased in study group compared with control group (P＜0.05).Biochemical pregnancy rate and clinical pregnancy rate were 1 6 .3 8% and 9 .05 % in study group ,which were significantly lower than 2 3 .1 7 % and 19.04% in control group,respectively (P＜0.05).Miscarriage rate of biochemical pregnancy in study group was 7. 33%,significantly higher than 4.13% in control group (P＜0.05).Corpus luteum V and vascular parameters in women with biochemical pregnancy abortion in both groups were significantly lower than those with clinical pregnancy (P＜0.05).Conclusion The decreased of blood supply of the corpus luteum in embryo implantation window of elderly women may be related to the reduction of endometrial receptivity and pregnancy rate.Regardless of old age,biochemical pregnancy abortion is likely to lead to loss of corpus luteum blood supply,which results in pregnancy failure.

Lynch Syndrome:Report of One Case and Literature Review / 中国医学科学院学报

This article reports a female type Ⅱ Lynch syndrome patient who suffered from colon cancer and endometrium carcinoma consecutively. We also briefly elucidate the pathogenesis,clinicopathological features,screening,and management of Lynch syndrome. Regular screening and early diagnosis and treatment may help to reduce the incidences and mortality rates of tumors associated with Lynch syndrome.

Aberrant Expressions of Immune Factors Facilitate the Disequilibrium of Immune Status in Cervical Cancer / 中国医学科学院学报

Objective To explore the expressions and co-relationship of immune factors forkhead box p3 (FoxP3),chemokine (C-C motif) ligand 22 (CCL22),tumor necrosis factor receptor superfamily member 40(OX40),and SMAD family member 3 (Smad3) in cervical carcinoma and investigate their immunomodulatory roles in cervical carcinogenesis.Methods Totally 30 cases of cervical carcinoma with adjacent tissues and 20 cases of normal cervix were collected in this study. FoxP3,CCL22,OX40,and Smad3 mRNA expressions were detected by real-time polymerase chain reaction (RT-PCR). Results Compared to normal cervix,the expression levels of FoxP3 and CCL22 mRNA were elevated in neoplastic foci(P=0.000,P=0.002) and tumor periphery (P=0.048,P=0.040).The mRNAs increased modestly in high-grade squamous cell carcinoma focal(P=0.019,P=0.020) and periphery tissue (P=0.023,P=0.031) in comparison with low-grade squamous cell carcinoma. The expression levels of OX40 and Smad3 mRNA were significantly lower in neoplastic foci(P=0.000,P=0.015) than normal cervix. Compared to low-grade squamous cell carcinoma focal and periphery tissue,the mRNAs decreased moderately in high-grade squamous cell carcinoma(P=0.018,P=0.030; P=0.027,P=0.014). In both neoplastic foci and tumor periphery,the mRNA expression level of CCL22 was positively correlated with FoxP3 (r=0.353,P=0.000; r=0.307,P=0.000) but negatively correlated with OX40 (r=-0.288,P=0.031; r=-0.263,P=0.037),while OX40 was positively correlated with Smad3 (r=0.384,P=0.002;r=0.288,P=0.023). The mRNA expressions of FoxP3 and CCL22 were increased in foci and pericarcinous tissues (P=0.024,P=0.039; P=0.032,P=0.034) while Smad3 was decreased in neoplastic foci (P=0.017) in contrast to HPV negative corresponding group. Conclusion FoxP3 and CCL22 expressions increase while OX40 and Smad3 expression decrease at mRNA level in the microenvironment of cervical cancer,which may be associated with such immunological model that the immunosuppressive roles of FoxP3 and CCL22 enhance while the immunity-boosting roles of OX40 and Smad3 are impeded,contributing to the deterioration of immune disequilibrium in local site and cervical cancer carcinogenesis.

Natural Outcome of Genital Tract High-risk Human Papillomavirus Infection and Associated Factors among 760 Women / 中国医学科学院学报

<p><b>OBJECTIVE</b>To investigate the natural outcome of genital tract high risk human papillomavirus (HR-HPV) infection and associated factors among women in Xi'an region.</p><p><b>METHODS</b>Totally 760 women with primary genital tract HR-HPV infection were enrolled and followed up by HPV-DNA genotyping technology. The cervical cytological techniques and/or colposcopy were used when necessary.</p><p><b>RESULTS</b>Among these subjects,the natural clearance rate of HR-HPV infection was 71.58%,with the median time of 8.10 months. The rate of HPV persistent infection was 22.63%, with the median time of 17.23 months. The rate of progression to cervical intraepithelial neoplasia (CIN)/cervical cancer (CC) was 5.79%, with the median time of 29.34 months. The natural clearance rate (P=0.000), persistent infection rate (P=0.000) and progression rate (P=0.040) in women older than 50 years were statistically difference from other age groups. The persistent infection rate in multiple infections group was significantly lower than that in single infection group (P=0.010), with the median time statistically longer than that in single infection group (P=0.018). The most easily progressive genotypes were HPV-16,HPV-33, HPV-58,HPV-18,HPV-52, and HPV-68, among which HPV-16 was the most common genotype in CIN 3/CC cases, accounting for 85.00%.</p><p><b>CONCLUSIONS</b>Most of HR-HPV infections are naturally cleared within 2 years, and only a few cases progresses to CIN/CC. Women older than 50 years have a lower natural clearance rate and higher persistent and progressive rates. Multiple infections can affect the persistent infection. HPV-16 is the most common carcinogenic genotype in Xi'an region.</p>

Screening of the genital human papillomavirus infection among 8581 women in the First Affiliated Hospital of Xi'an Jiaotong University / 中国医学科学院学报

<p><b>OBJECTIVE</b>To investigate the prevalence of genital human papillomavirus (HPV) infection among women in Shaan'xi Province of China, and to detect the characteristics of genital HPV infection among these women.</p><p><b>METHODS</b>Totally 8581 women in the Department of Gynaecology of the First Affiliated Hospital of Xi'an Jiaotong University were enrolled to detect HPV-DNA in their genital tracts by PCR+flow-through hybridization. Some subjects were also screened by cytology or histology.</p><p><b>RESULTS</b>Among these subjects, the prevalence of female genital tract HPV infection was 33.06%, among which high risk (HR)-HPV infection accounted for 30.08% and multiple HR-HPV infection 27.18%. The genotypes HPV-16, -52,-58, -6, and HPV-18 were the most prevalent strains. The distribution of HPV infection differed among different age groups:the first infection peak occured less than 25 years and the second peak was above 51 years. Among the HR-HPV DNA positive women, the rates of HR-HPV infection with normal histology, cervical intraepithelial neoplasias 1 (CIN1), CIN2 ,CIN3, and cervical cancer (CC) were 88.72%, 97.48%, 97.35%, 95.88%, and 99.23%, respectively. For women with CC, HPV-16 was the most common genotype, followed by HPV-18, HPV-58, HPV-52, and HPV-33. HPV-16 ,-18, and 58 were responsible for 94.62% of CC cases in this region.</p><p><b>CONCLUSIONS</b>The most common genotypes of HPV infection among women in Shaan'xi province are HPV-16, HPV-52, HPV-58, HPV-6, and HPV-18. HPV-16, HPV-18, HPV-58, HPV-52, and HPV-33 are the most frequently genotypes found in cervical carcinoma, in particular HPV-16, HPV-18, and HPV-58. HR-HPV infection aggravates the cervical lesions. The multiple HPV infections increase the risk of cervical lesions.</p>

Clinical investigation on intravesical instillation of high-dose pirarubicin during perioperative period to prevent bladder carcinoma recurrence / 中华外科杂志

<p><b>OBJECTIVE</b>To evaluate the efficacy and safety of intravesical instillation of high-dose pirarubicin during perioperative period to prevent bladder carcinoma recurrence.</p><p><b>METHODS</b>A prospective randomized controlled clinical trial was carried out. A total of 120 patients with bladder transitional cell carcinoma (TNM I-II) underwent partial cystectomy or transurethral resection of bladder tumour were randomly divided into 2 groups, which were chosen from the patients were hospitalized during June 2003 to May 2009. There were 62 patients were in group A and 58 patients were in group B. In group A, intravesical instillation of high-dose pirarubicin during perioperative period was conducted. In group B, intravesical perfusion of pirarubicin was performed regularly after operation. All the patients were followed up in order to record the relapse rates and adverse reaction.</p><p><b>RESULTS</b>No significant difference was found between group A and group B from gender, age, pathological stage and operation methods (P > 0.05). The recurrence rates were 8.1% in group A, and 20.7% in group B. The adverse effect rates of urethrostenosis in group A and group B were 3.2% and 18.9%. The adverse effect rates of cystic stimulation including irritation signs of bladder and hematuria in group A and group B were 4.8% and 27.6%. There were significant differences in recurrence rates and adverse effect rates between the two groups (P > 0.05).</p><p><b>CONCLUSION</b>Intravesical instillation of high-dose pirarubicin during perioperative period is an effective procedure for the prevention of bladder cancer recurrence.</p>

Human chorionic gonadotrophin regulates epidermal growth factor in the phallus of hypospadias mice / 中华男科学杂志

<p><b>OBJECTIVE</b>To investigate the etiopathogenesis of congenital hypospadias and the regulating effect of exogenous human chorionic gonadotrophin (hCG) on the epidermal growth factor (EGF) in the phallus of hypospadiac mice.</p><p><b>METHODS</b>Mouse models of congenital hypospadias were established. Fifty healthy male mice randomly selected as normal controls received intraperitoneal injection of normal saline, and another 50 with hypospadias were equally divided into an experimental control group, intraperitoneally injected with 1 ml normal saline, and 4 hCG dose groups treated by hCG intraperitoneal injection at 50 IU, 100 IU, 150 IU and 200 IU respectively for 7 consecutive days. The concentrations of EGF in the phallus and serum were detected in different groups by ELISA.</p><p><b>RESULTS</b>The concentrations of EGF in the phallus were significantly lower in the hypospadias rats than in the normal controls (P < 0.05), the 150 IU and 200 IU hCG groups showing significant differences from the 50 IU, 100 IU and experimental control groups (P < 0.05), as the 50 IU, 100 IU and experimental control groups from the normal control (P < 0.05). But no obvious difference was found in EGF concentration in the serum among different groups (P > 0.05).</p><p><b>CONCLUSION</b>Nonsteroidal antiandrogen and decreased concentration of EGF in the mouse phallus may be associated with the etiology of hypospadias. And exogenous hCG at 150 IU or 200 IU can increase the concentration of EGF in the phallus of mice with congenital hypospadias.</p>

Efficacy of large decompressive craniectomy in severe traumatic brain injury / 中华创伤杂志(英文版)

<p><b>OBJECTIVE</b>To investigate the role of large decompressive craniectomy (LDC) in the management of severe and very severe traumatic brain injury (TBI) and compare it with routine decompressive craniectomy (RDC).</p><p><b>METHODS</b>The clinical data of 263 patients with severe TBI (GCS < or = 8) treated by either LDC or RDC in our department were studied retrospectively in this article. One hundred and thirty-five patients with severe TBI, including 54 patients with very severe TBI (GCS < or = 5), underwent LDC (LDC group). The other 128 patients with severe TBI, including 49 patients with very severe TBI, underwent RDC (RDC group). The treatment outcome and postoperative complications of the two treatment methods were compared and analyzed in a 6-month follow-up period.</p><p><b>RESULTS</b>Ninety-six patients (71.7 %) obtained satisfactory treatment outcome in the LDC group, while only 75 cases (58.6 %) obtained satisfactory outcome in the RDC group (P < 0.05). Moreover, the efficacy of LDC in treating very severe TBI was higher than that of RDC (63.0 % vs. 36.7 %, P < 0.01). The chance of reoperation due to refractory intracranial pressure (ICP) in the LDC group was significantly lower than that of the RDC group (P < 0.05), while the incidences of delayed intracranial hematoma and subdural effusion were significantly higher than those of the RDC group ( P < 0.05).</p><p><b>CONCLUSIONS</b>LDC is superior to RDC in improving the treatment outcome of severe TBI, especially the very severe ones. LDC can also efficiently reduce the chances of reoperation due to refractory ICP. However, it increases the incidences of delayed intracranial hematoma and contralateral subdural effusion.</p>

Inhibitory effect of progesterone on inflammatory factors after experimental traumatic brain injury / 生物医学与环境科学（英文）

<p><b>OBJECTIVE</b>Traumatic brain injury (TBI) is one of the leading causes of morbidity and mortality in young people. Inflammatory cytokines play an important part in the pathophysiology of TBI. Recent studies demonstrate that progesterone significantly reduces cerebral edema and enhances functional recovery from TBI and stroke in several animal models. This study was designed to investigate the inhibitory effect of progesterone on inflammatory response after traumatic brain injury.</p><p><b>METHODS</b>Progesterone was injected intraperitoneally using rats as a model of traumatic brain injury, and Western blot technique was applied to detect the expression of three inflammation-related factors: nuclear factor kappa B p65 (NFkappaB p65), glial fibrillary acidic protein (GFAP), and tumor necrosis factor-alpha (TNF-alpha). The water content of injured brain was also examined. A neurological severity score was recorded to evaluate the effect of progesterone on neurodeficit recovery.</p><p><b>RESULTS</b>NFkappaB p65, GFAP, and TNF-alpha were increased in all injured animals. In rats treated with progesterone, the expression level of NFkappaB p65 and TNF-alpha were reduced significantly in comparison with vehicle-treated rats. However, progesterone did not alter the expression of GFAP in the injured rats. Progesterone also reduced the water content of injured brain and the lesion volume. In addition, progesterone-treated injured rats showed significant improvements in the Neurological Severity Score test, compared with vehicle-treated ones.</p><p><b>CONCLUSIONS</b>Progesterone inhibits the inflammatory response after experimental traumatic brain injury and mitigates the severity of brain damage.</p>

Surgery for posttraumatic syringomyelia: a retrospective study of seven patients / 中华创伤杂志(英文版)

<p><b>OBJECTIVE</b>To analyze retrospectively the clinical symptoms, signs, radiological findings and results of treatment of posttraumatic syringomyelia.</p><p><b>METHODS</b>The data of 7 patients with posttraumatic syringomyelia confirmed by computerized tomography (CT) and magnetic resonance imaging (MRI) in our hospital between 1999 and 2004 were reviewed retrospectively. The patients underwent decompressive laminectomy or syringo-subarachnoid (S-S) shunting with microsurgery. Long-term follow-up was available (range: 13-65 months).</p><p><b>RESULTS</b>The major clinical manifestations of posttraumatic syringomyelia usually included the onset of increasing signs and the development of new symptoms after an apparently stable period. The clinical symptoms included pain, sensory disturbance, weakness, and problems in autonomic nerves. Syrinx existed merely at the cervical level in 4 cases and extended downward to the thoracic levels in the other 3 cases. One case underwent decompressive laminectomy, 6 cases were treated by S-S shunting. During the early postoperative period, all the patients showed an improvement of symptoms of syrinx without major complication or death. The decreased size or collapse of the syrinx was demonstrated by postoperative MRI.</p><p><b>CONCLUSIONS</b>Posttraumatic syringomyelia is a disabling sequela of spinal cord injury, developing months to years after spinal injury. MRI is the standard diagnostic technique for syringomyelia. The patients with posttraumatic syringomyelia combined with progressive neurological deterioration should be treated with operations. S-S shunting procedure is effective in some patients with posttraumatic syringomyelia. Decompressive procedure may be an alternative primary surgical treatment for patients with kyphosis and cord compression.</p>

Impact of the hCG on epidermal growth factor in the phallic tissues of hypospadiac mice / 中华男科学杂志

<p><b>OBJECTIVE</b>To investigate the impact of hCG on the epidermal growth factor (EGF) in the phallic tissues of hypospadiac mice.</p><p><b>METHODS</b>The Kunming mouse model of congenital hypospadias was established. As controls, 10 three-week-old normal male mice and 10 3-week-old congenital hypospadiac mice were injected with isometric sodium chloride intraperitoneally. Another 40 mice with hypospadias were divided into 4 groups and injected intraperitoneally with 50 IU, 100 IU, 150 IU and 200 IU of hCG respectively per day for 7 days. Then the concentration of ECG in the phallus and in the serum were assayed by ELISA.</p><p><b>RESULTS</b>The EGF level in the phallus of the hypospadiac mice was much lower than that of the controls statistically (P < 0. 05). After the injection of exogenous hCG, the concentration of ECG increased with the dose of hCG, 50 IU (59.57 +/- 22.16) pg/ml, 100 IU (57.97 +/- 9.59) pg/ml, 150 IU (73.02 +/- 31.35) pg/ml and 200 IU (92.45 +/- 35.74) pg/ml. The concentration of ECG showed no significant difference between the control group and the 150 IU and 2000 IU groups (P > 0.05).</p><p><b>CONCLUSION</b>Decreased concentration of EGF in the mouse phallus may be associated with the pathogeny of hypospadias. A proper dose of exogenous hCG may increase the concentration of EGF in the mouse phallus.</p>

Identification of metastasis-associated proteins of ovarian cancer by proteomics / 中华病理学杂志

<p><b>OBJECTIVE</b>To investigate the differenal protein expression profiles of ovarian tumor cell lines with distinct metastatic abilities.</p><p><b>METHODS</b>The ovarian cancer cell line HO8910 and HO8910pm, derived from same parental cells but exhibited different metastatic ability, were investigated by two-dimensional gel electrophoresis (2-DE)-MALDI-TOF-MS proteomic approach.</p><p><b>RESULTS</b>Thirty-nine proteins were detected by 2-DE to have expression disparity levels over 2 folds between two cell lines. Eighteen of them were identified by MALDI-TOF-MS. The proteins are involved in apoptosis, extra cellular matrix (ECM), cytoskeleton, growth factor, glycolysis, protein metabolism and immune system.</p><p><b>CONCLUSION</b>The data are valuable for the identification of differentially expressed proteins involved in the biological behavior of human ovarian cancer.</p>

Clinical study on the improvement of ischemia condition with stem cell transplantation in 122 cases necrosis of femoral head / 中华外科杂志

<p><b>OBJECTIVE</b>To observe the curative effects of bone marrow stem cell (BMSC) and peripheral blood stem cell (PBSC) transplantations on the avascular necrosis of femoral head (ANFH).</p><p><b>METHODS</b>Totally 122 ANFH patients (211 coxae) treated by BMSC or PBSC transplantations were enrolled from July 2004 to December 2006. All of them were classed to different stages according to the ARCO. Control group were desired as themselves before and after treatment. The puncture of femoral artery was conducted with digital subtraction angiography (DSA), and the tubes were inserted into medial femoral circumflex artery, lateral femoral circumflex artery and obturator artery with the cell suspensions were gradually poured into the arteries.</p><p><b>RESULTS</b>The joint pain, joint functions and walking distance of 122 patients were detected for the follow-up. Compared with before treatment, the calibers thickened; vessels increased and blood velocity quickened of femoral head blood-supply artery were observed in 15 patients after 6 months checked by DSA. The reduced areas of femoral head necrosis in 8 patients indicated the new bone formation between 12 and 24 months.</p><p><b>CONCLUSIONS</b>Autologous BMSC and PBSC transplantation results in the new bone formation and improvement of ischemia in areas of femoral head necrosis at 6 months. The change of angiography was observed about 12 to 24 months after cell transplantation. The stem cell transplantation is convenient, safe and effective in the treatment of the ANFH with no adverse reaction, and can be considered as a new therapy of ANFH.</p>

New principles in tumor antigens and their significance in future immunotherapies for lymphomas and other malignancies--editorial / 中国实验血液学杂志

The molecular characterization of self-antigens expressed by human malignancies that are capable of elicitation of anti-tumor immune responses in patients has been an active field in hematology, oncology, and tumor immunology. More than 2000 tumor antigens have been identified. These significant progresses have led to the renaissance of tumor immunology and studies on novel anti-tumor immunotherapies in lymphomas, other hematologic malignancies and tumors. However, despite of the progress in the identification of these self-tumor antigens, current antigen-specific immunotherapies for tumors are far less satisfactory than that expected, which reflects the urgent need to improve our understanding on the basic principles underlying the selection of these self-tumor antigens. In order to develop more effective antigen-specific anti-tumor immunotherapies and to monitor the responses to these immunotherapies in patients with lymphomas and other malignancies, many additional questions need to be addressed. In this brief review, the progress in the identification of tumor antigens in lymphomas and other malignancies was outlined and the new principles of self-tumor antigens and their significance for future immunotherapies to these malignancies were summarized.

A DSP-based design method for detecting fetal heart rate signals / 中国医疗器械杂志

The paper presents the development and design of an ultrasonic Doppler fetal heart rate monitoring system based on DSP, and explains the realization of a algorithm for detecting fetal heart rate. Clinical practice has proved that this system is able to pick up the real-time fetal heart rate correctly and rapidly.